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  2. Clean Water Act Analytical Methods
  3. Approved Microbiological Methods
  4. Wastewater & Sewage Sludge

Approved CWA Microbiological Methods for Ambient Water

Methods approved under Clean Water Act Section 304(h) and published at 40 CFR Part 136.

  • Method 1623.1: Cryptosporidium and Giardia in Water by Filtration/IMS/FA (pdf) (1020.91 KB, January 2012, EPA 816-R-12-001)
  • 1103.2: Escherichia coli (E. coli) in Water by Membrane Filtration Using membrane-Thermotolerant Escherichia coli Agar (mTEC) (pdf) (1.26 MB, September 2023, EPA-821-R-23-009)
  • 1106.2: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus-Esculin Iron Agar (mE-EIA) (pdf) (1.19 MB, September 2023, EPA-821-R-23-007)
  • 1600.1: Enterococci in Water by Membrane Filtration Using membrane-Enterococcus Indoxyl-β-D-Glucoside Agar (mEI) (pdf) (1020.29 KB, September 2023, EPA-821-R-23-006)
  • 1603.1: Escherichia coli (E. coli) in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar (Modified mTEC) (pdf) (957.93 KB, September 2023, EPA-821-R-23-008)
  • 1604: Total Coliforms and Escherichia coli in Water by Membrane Filtration Using a Simultaneous Detection Technique (MI Medium) (pdf) (383.57 KB, September 2002, 821-R-02-024)
  • 1622: Cryptosporidium in Water by Filtration/IMS/FA (pdf) (1.01 MB, December 2005, 815-R-05-001)
  • 1623: Cryptosporidium and Giardia in Water by Filtration/IMS/FA (pdf) (937.09 KB, December 2005, 815-R-05-002)

Clean Water Act Analytical Methods

  • PFAS Methods
  • Methods Update Rules
  • Approved Chemical Methods
    • Inorganic Non‑Metals
    • Metals
    • Organic
  • Approved Microbiological Methods
    • Wastewater & Sewage Sludge
    • Ambient Water
  • Approved WET Methods
    • Acute
    • Chronic - Freshwater
    • Chronic - Marine/Estuarine
  • Approved Radiochemical Methods
  • Approved Industry-Specific Methods
  • Other CWA Methods: Chemical
  • Other CWA Methods: Microbiological
  • Other CWA Methods: Biosolids
  • Alternate Test Procedures
  • Regulatory History
Contact Us about CWA Analytical Methods
Contact Us about CWA Analytical Methods to ask a question, provide feedback, or report a problem.
Last updated on June 19, 2024
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